Journal: RSC Advances

Article Title: Electrochemical evaluation of screen-printed sensor manufacturing and LOx enzyme immobilization for lactate biomarker detection: influence of reference electrode material

doi: 10.1039/d4ra06473a

Figure Lengend Snippet: Lactate oxidation reaction. The reaction shows the oxidation of lactate to pyruvate by lactate oxidase (LOx) in the presence of oxygen (O 2 ), generating hydrogen peroxide (H 2 O 2 ) as a byproduct.

Article Snippet: Nafion 117 (5%, Sigma-Aldrich) and Lactate Oxidase (LOx) Enzyme from a commercial kit (Labtest® Kit) suspended in 50 mmol per L buffer pH 7.2, 4-aminoantipyrine ≥ 0.05 mmol per L peroxidase ≥ 1000 U L −1 and preservatives, lactate oxidase ≥ 1000 U L −1 , N -ethyl- N -(2-hydroxy-3-sulfopropyl)-3-methyllaniline (TOOS) ≥ 1.5 mmol L −1 and preservatives were used to modify the sensors.

Techniques:

Journal: RSC Advances

Article Title: Electrochemical evaluation of screen-printed sensor manufacturing and LOx enzyme immobilization for lactate biomarker detection: influence of reference electrode material

doi: 10.1039/d4ra06473a

Figure Lengend Snippet: Review of printed sensors for enzymatic detection of the lactate analyte. NR: not reported. HRP: horseradish peroxidase

Article Snippet: Nafion 117 (5%, Sigma-Aldrich) and Lactate Oxidase (LOx) Enzyme from a commercial kit (Labtest® Kit) suspended in 50 mmol per L buffer pH 7.2, 4-aminoantipyrine ≥ 0.05 mmol per L peroxidase ≥ 1000 U L −1 and preservatives, lactate oxidase ≥ 1000 U L −1 , N -ethyl- N -(2-hydroxy-3-sulfopropyl)-3-methyllaniline (TOOS) ≥ 1.5 mmol L −1 and preservatives were used to modify the sensors.

Techniques: Modification, Encapsulation, Membrane

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Inflammasome priming by LPS is dependent upon ERK signaling and proteasome function *

doi: 10.4049/jimmunol.1301974

Figure Lengend Snippet: Monocytes were nucleofected with ERK1 and ERK2 specific siRNA or scrambled control and recovered overnight. Cells were then primed with LPS (1μg/ml) for 30 min and activated with ATP (5mM) for another 30 min. Supernatants were analyzed for IL-18 release and cell lysates were immunoblotted to confirm ERK protein down regulation using total ERK antibody. IL-18 ELISA represents the mean± SEM for three separate monocyte donors and the blots are representative of repeated blots. **p < 0.01 as compared to scrambled siRNA.

Article Snippet: In knockdown experiments, small interfering RNA (siRNA) against ERK1 and scrambled siRNA were purchased from Sigma-Aldrich while Signalsilencer ® p42 MAPK (ERK2) siRNA II was purchased from Cell Signaling Company. siRNAs were delivered in monocytes by Amaxa nucleofector I (Lonza).

Techniques: Enzyme-linked Immunosorbent Assay